Identification of β-Lactamase in Antibiotic-Resistant Bacillus cereus Spores

Author:

Fenselau Catherine1,Havey Crystal1,Teerakulkittipong Nuttinee1,Swatkoski Stephen1,Laine Olli1,Edwards Nathan2

Affiliation:

1. Department of Chemistry and Biochemistry

2. Center for Bioinformatics and Computational Biology, University of Maryland, College Park, Maryland 20742

Abstract

ABSTRACT β-Lactamase type I is reported for the first time to occur in the sporulated form in a penicillin-resistant Bacillus species. The enzyme was readily characterized from the B. cereus 5/B line (ATCC 13061) by mass spectrometry and two-dimensional gel electrophoresis.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference20 articles.

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3. Jagtap, P., G. Michailidis, R. Zielke, A. K. Walker, N. Patel, J. R. Strahler, A. Driks, P. C. Andrews, and J. R. Maddock. 2006. Early events of Bacillus anthracis germination identified by time-course quantitative proteomics. Proteomics6:5199-5211.

4. Jenkinson, H. F., W. D. Sawyer, and J. Mandelstam. 1981. Synthesis and order of assembly of spore coat proteins in Bacillus subtilis. J. Gen. Microbiol.123:1-16.

5. Jensen, O. N., M. Wilm, A. Shevchenko, and M. Mann. 1999. Sample preparation methods for mass spectrometric peptide mapping directly from 2-DE gels. Methods Mol. Biol.112:513-530.

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