Affiliation:
1. Department of Molecular Genetics, The Ohio State University, Columbus, Ohio
Abstract
ABSTRACT
A method to rapidly generate gene replacement constructs by fusion PCR is described for
Aspergillus nidulans
. The utility of the approach is demonstrated by green fluorescent protein (GFP) tagging of
A. nidulans ndc80
to visualize centromeres through the cell cycle. The methodology makes possible large-scale GFP tagging, promoter swapping, and deletion analysis of
A. nidulans
.
Publisher
American Society for Microbiology
Subject
Molecular Biology,General Medicine,Microbiology
Cited by
231 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献