Rapid Fluorescent-Antibody Stain Technique with Group A Streptococci

Author:

Freeburg Pat W.1

Affiliation:

1. Laboratory Department, Northeast Colorado Health Department, Sterling, Colorado 80751

Abstract

A rapid fluorescent-antibody stain technique used with young broth cultures of trypsinized group A streptococci is described. Results from 1,214 trypsinized cultures of group A streptococci employing the rapid stain method were equivalent, both in specificity and sensitivity, when compared to results obtained from identical non-trypsinized cultures stained with the longer standard technique of Cherry, Goldman, and Carski. Moreover, the rapid method requires less than 2 min to complete.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference15 articles.

1. Fluorescent antibody techniques in the diagnosis of communicable diseases;Cherry W. B.;U. S. Pub. Health Serv. PubI.,1960

2. Localization of antigen in tissue cells. II. Improvements in a method for the detection of antigen by means of fluorescent antibody;Coons A. H.;J. Exp. Med.,1950

3. Effects of trypsin on the permeability of streptococci;Fox E. N.;J. Bacteriol.,1960

4. Use of a rapid fluorescent-antibody staining technique with group A streptococci;Freeburg P. W.;Appl. Microbiol.,1969

5. Studies of streptococcal cell walls. III. The amino acids of the trypsin-treated cell wall;Hayashi J. A.;J. Bacteriol.,1959

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