Rapid fluorescent-antibody method with bromelase for identification of group A streptococci

Author:

Waters C A,Makens M A

Abstract

This is a preliminary report on a rapid fluorescent-antibody staining method utilizing the enzyme Bromelase that effectively eliminates nonspecific staining of staphylococci. By this simple method, group A streptococci were accurately identified from 2- to 4-h broth cultures.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference13 articles.

1. Fluorescentantibody techniques in diagnostic bacteriology;Cherry W. B.;Bacteriol. Rev.,1965

2. Simplified fluorescent-antibody staining method for primary plate isolates of group A streptococci;Ederer G. M.;Appl. Microbiol.,1972

3. Comparison of fluorescent antibody, precipitin, and bacitracin disk methods in the identification of group A streptococci;Estela L. A.;Am. J. Clin. Pathol.,1963

4. Facklam R. R. 1974. Streptococci p. 96-108. In E. H. Lennette E. H. Spaulding and J. P. Truant (ed.) Manual of clinical microbiology 2nd ed. American Society for Microbiology Washington D.C.

5. Role of protein A in nonspecific immunofluorescence of Staphylococcus aureus;Forsgren A.;Infect. Immun.,1970

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