Immunofluorescent Cell-Counting Assay for Lymphocytic Choriomeningitis Virus-Reference-Cited by-同舟云学术

Immunofluorescent Cell-Counting Assay for Lymphocytic Choriomeningitis Virus

Published:1974-07 Issue:1 Volume:28 Page:17-21
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Webster Janice M.¹,Kirk B. E.¹

Affiliation:

1. Department of Microbiology, West Virginia University Medical Center, Morgantown, West Virginia 26506

Abstract

A quantitative assay for lymphocytic choriomeningitis virus was developed and standardized. The assay is based on direct immunofluorescent staining of infected L-929 cell monolayers and enumeration of cells containing fluorescent viral antigens. Maximal adsorption of virus to cells occurred within 1 h. Observations on the sequential development of viral antigens within cells showed that specific cytoplasmic fluorescence appeared within 10 h. The optimal time for enumerating fluorescent cells was from 18 to 20 h after addition of virus. A linear relationship was demonstrated between the number of infected cells and the relative virus concentration. Fluorescent cells were distributed randomly in infected cover slip cell monolayers. The immunofluorescent cell-counting assay for lymphocytic choriomeningitis virus was highly precise and reproducible.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.28.1.17-21.1974

Reference17 articles.

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3. Carter G. B. 1973. Immunofluorescence in the rapid identification of viruses and the role of fluorescent cell counting p. 54-65. In F. J. Baker (ed.) Microbiology of the seventies. Butterworths London.

4. Localization of antigen in tissue cells. II. Improvements in a method for the detection of antigen by means of fluorescent antibody;Coons A. H.;J. Exp. Med.,1950

5. Fluorescence assay of foamy virus;Fleming W. A.;J. Gen. Virol.,1970

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