Affiliation:
1. Department of Microbiology, West Virginia University Medical Center, Morgantown, West Virginia 26506
Abstract
A 24-h neutralization test that is based on fluorescent cell counting was used for the detection and quantitative determination of serum-neutralizing antibody against lymphocytic choriomeningitis virus. The earliest manifestation of serum-neutralizing antibody in guinea pigs was shown to occur within 1 week after inoculation with lymphocytic choriomeningitis virus. Within 11 weeks, serum-neutralizing antibody increased from 20- to 300-fold. Neutralizing end points of serum samples, obtained from 3 through 11 weeks, were enhanced as much as sevenfold by complement. Anti-guinea pig immunoglobulin G or anti-whole guinea pig serum potentiated the neutralizing activity of serum as much as 20- and 40-fold, respectively.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference14 articles.
1. Antibody affinity and valence in viral neutralization;Blank S. E.;J. Immunol.,1972
2. Casey H. 1965. Standardized diagnostic complement fixation method and adaptation to micro test. In Public Health Service Monogr. no. 74 Washington D.C.
3. Neutralization anti-IgG test for antisera to Venzuelan equine encephalomyelitis;Hahon N.;J. Gen. Virol.,1970
4. Heremans J. F. and J. P. Vaerman. 1971. Biological significance of IgA antibodies in serum and secretions p. 875-890 In B. Amos (ed.) Progress in immunology. Academic Press Inc. New York.
5. Pouziti immunofluorescence k detekci protilatek a vizualizaci antigenu u Iymfocytarni choriomenigity;Hronovsky V.;Cesk. Epidemiol. Mikrobiol. Imunol.,1969
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