Hyaluronic Acid Production in Bacillus subtilis

Author:

Widner Bill1,Behr Régine1,Von Dollen Steve1,Tang Maria1,Heu Tia1,Sloma Alan1,Sternberg Dave1,DeAngelis Paul L.2,Weigel Paul H.2,Brown Steve1

Affiliation:

1. Novozymes, Inc., 1445 Drew Avenue, Davis, California 95616

2. Department of Biochemistry and Molecular Biology and the Oklahoma Center for Medical Glycobiology, University of Oklahoma Health Sciences Center, 940 Stanton L. Young Blvd., Oklahoma City, Oklahoma 73104

Abstract

ABSTRACT The hasA gene from Streptococcus equisimilis , which encodes the enzyme hyaluronan synthase, has been expressed in Bacillus subtilis , resulting in the production of hyaluronic acid (HA) in the 1-MDa range. Artificial operons were assembled and tested, all of which contain the hasA gene along with one or more genes encoding enzymes involved in the synthesis of the UDP-precursor sugars that are required for HA synthesis. It was determined that the production of UDP-glucuronic acid is limiting in B. subtilis and that overexpressing the hasA gene along with the endogenous tuaD gene is sufficient for high-level production of HA. In addition, the B. subtilis -derived material was shown to be secreted and of high quality, comparable to commercially available sources of HA.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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