Bioluminescent Most-Probable-Number Method To Enumerate lux -Marked Pseudomonas aeruginosa UG2Lr in Soil-Reference-Cited by-同舟云学术

Bioluminescent Most-Probable-Number Method To Enumerate lux -Marked Pseudomonas aeruginosa UG2Lr in Soil

Published:1994-09 Issue:9 Volume:60 Page:3458-3461
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Flemming C. A.¹,Lee H.¹,Trevors J. T.¹

Affiliation:

1. Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W1

Abstract

Bioluminescence measured with a luminometer and charge-coupled device was an effective marker in most-probable-number assays for luxAB -marked Pseudomonas aeruginosa UG2Lr in soil. Most-probable-number assays with microtiter plate wells and luminometer tubes gave estimates for UG2Lr that were similar to viable colony counts. Both methods detected five cells per g of soil.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.60.9.3458-3461.1994

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