Affiliation:
1. Department of Periodontology, Oral Microbiology Testing Laboratory, School of Dentistry,1 and
2. Department of Periodontology, Universidad del Valle, Cali, Colombia2
3. Department of Preventive Medicine, Division of Biostatistics, School of Medicine,3 University of Southern California, Los Angeles, California, and
Abstract
ABSTRACT
Anaerobic culture is employed routinely in the primary isolation of periodontal pathogenic bacteria. However, little or no data exist on the relative abilities of the Coy anaerobic chamber (Coy Laboratory Products, Grass Lake, Mich.), the GasPak (Becton Dickinson Microbiology Systems, Cockeysville, Md.), and the AnaeroPack (Mitsubishi Gas Chemical America, Inc., New York, N.Y.) systems to grow important periodontal species, including
Porphyromonas gingivalis
,
Prevotella intermedia/nigrescens
,
Bacteroides forsythus
,
Eubacterium
species,
Campylobacter
species,
Fusobacterium
species, and
Peptostreptococcus micros
. A total of 78 specimens from advanced periodontitis lesions were collected anaerobically, plated on enriched blood agar medium, and incubated at 35°C for 5 to 7 days in each anaerobic culture system. The three culture systems were equally efficient in isolating
Porphyromonas gingivalis
and
Prevotella intermedia/nigrescens
. The Coy anaerobic chamber yielded the highest proportional recoveries of
Campylobacter
(
P
= 0.0001; nonparametric analysis of variance) and
Eubacterium
(
P
= 0.009). The Coy anaerobic chamber and the GasPak system demonstrated higher proportional recoveries of
Bacteroides forsythus
(
P
= 0.0006) and
Peptostreptococcus micros
(
P
= 0.0001) than the AnaeroPack system. The AnaeroPack system was most efficient in growing
Fusobacterium
species (
P
= 0.0001). Overall, the Coy anaerobic chamber and the GasPak system showed the highest proportional recoveries of putative periodontal pathogens, but the recoveries by the various anaerobic test systems varied considerably from sample to sample.
Publisher
American Society for Microbiology
Cited by
29 articles.
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