Rapid PCR Detection of Staphylococcus aureus Clonal Complex 398 by Targeting the Restriction-Modification System Carrying sau1-hsdS1-Reference-Cited by-同舟云学术

Rapid PCR Detection of Staphylococcus aureus Clonal Complex 398 by Targeting the Restriction-Modification System Carrying sau1-hsdS1

Published:2011-02 Issue:2 Volume:49 Page:732-734
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Stegger Marc¹,Lindsay Jodi A.²,Moodley Arshnee³,Skov Robert¹,Broens Els M.⁴⁵,Guardabassi Luca³

Affiliation:

1. Department of Microbiological Surveillance and Research, Statens Serum Institut, 5 Artillerivej, DK-2300 Copenhagen S, Denmark

2. Centre for Infection, Department of Cellular & Molecular Medicine, St George's, University of London, Cranmer Terrace, London SW17 0RE, United Kingdom

3. Department of Disease Biology, Faculty of Life Sciences, University of Copenhagen, 4 Stigbøjlen, DK1870 Frederiksberg C, Denmark

4. Quantitative Veterinary Epidemiology Group, Wageningen Institute of Animal Sciences, Wageningen University, P.O. Box 338, 6700 AH Wageningen, Netherlands

5. Centre for Infectious Disease Control Netherlands, National Institute for Public Health and the Environment, P.O. Box 1, 3720BA, Bilthoven, Netherlands

Abstract

ABSTRACT A PCR targeting sau1-hsdS1 was developed for rapid detection of Staphylococcus aureus clonal complex 398 (CC398). High sensitivity (100%) and specificity (100%) were shown by evaluating the test on a large strain collection ( n = 1,307). We recommend this test for accurate, rapid, and inexpensive diagnosis of methicillin-resistant S. aureus (MRSA) CC398 in hospitals and on farms.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01970-10

Reference12 articles.

1. Presence of a Novel DNA Methylation Enzyme in Methicillin-Resistant Staphylococcus aureus Isolates Associated with Pig Farming Leads to Uninterpretable Results in Standard Pulsed-Field Gel Electrophoresis Analysis

2. Rapid determination of hospital-acquired meticillin-resistant Staphylococcus aureus lineages;Cockfield J. D.;J. Med. Microbiol,2007

3. Analysis of the baseline survey on the prevalence of methicillin-resistantStaphylococcus aureus(MRSA) in holdings with breeding pigs, in the EU, 2008 - Part A: MRSA prevalence estimates

4. eBURST: Inferring Patterns of Evolutionary Descent among Clusters of Related Bacterial Genotypes from Multilocus Sequence Typing Data

5. Typing of Methicillin-Resistant Staphylococcus aureus in a University Hospital Setting by Using Novel Software for spa Repeat Determination and Database Management

Cited by 100 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Optimization fnbA gene target for potential detection Staphylococcus aureus as foodborne pathogen bacteria using Polymerase Chain Reaction method;PROCEEDINGS OF THE 2ND INTERNATIONAL INTERDISCIPLINARY SCIENTIFIC CONFERENCE “DIGITALIZATION AND SUSTAINABILITY FOR DEVELOPMENT MANAGEMENT: ECONOMIC, SOCIAL, AND ENVIRONMENTAL ASPECTS”;2024

2. Prevalence, antimicrobial resistance, and genetic lineages of nasal Staphylococcus aureus among medical students at a Spanish University: detection of the MSSA-CC398-IEC-type-C subclade;Research in Microbiology;2023-12

3. Nasal staphylococci microbiota and resistome in healthy adults in La Rioja, northern Spain: High frequency of toxigenic S. aureus and MSSA-CC398 subclade;Infection, Genetics and Evolution;2023-12

4. RdJ detection tests to identify a unique MRSA clone of ST105-SCCmecII lineage and its variants disseminated in the metropolitan region of Rio de Janeiro;Frontiers in Microbiology;2023-11-20

5. Transmission of livestock-associated methicillin-resistant Staphylococcus aureus between animals, environment, and humans in the farm;Environmental Science and Pollution Research;2023-07-07