Presence of a Novel DNA Methylation Enzyme in Methicillin-Resistant Staphylococcus aureus Isolates Associated with Pig Farming Leads to Uninterpretable Results in Standard Pulsed-Field Gel Electrophoresis Analysis

Author:

Bens Corina C. P. M.1,Voss Andreas1,Klaassen Corné H. W.1

Affiliation:

1. Department of Medical Microbiology and Infectious Diseases, Regional Public Health Laboratory, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands

Abstract

ABSTRACT Genomic DNA from methicillin-resistant Staphylococcus aureus isolates recovered from pigs and their caretakers proved resistant to SmaI digestion, leading to uninterpretable results in standard pulsed-field gel electrophoresis. This is the result of a yet unknown restriction/methylation system in the genus Staphylococcus with the recognition sequence CCNGG.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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