One-Step Identification of Five Prominent Chicken Salmonella Serovars and Biotypes

Author:

Zhu Chunhong1,Yue Min1,Rankin Shelley1,Weill François-Xavier2,Frey Joachim3,Schifferli Dieter M.1

Affiliation:

1. Department of Pathobiology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, Pennsylvania, USA

2. Institut Pasteur, Unité des Bactéries Pathogènes Entériques, Paris, France

3. Institute of Veterinary Bacteriology, Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland

Abstract

ABSTRACT Based on bacterial genomic data, we developed a one-step multiplex PCR assay to identify Salmonella and simultaneously differentiate the two invasive avian-adapted S. enterica serovar Gallinarum biotypes Gallinarum and Pullorum, and the most frequent, specific, and asymptomatic colonizers of chickens, serovars Enteritidis, Heidelberg, and Kentucky.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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