Sugar Utilization and Acid Production by Free and Entrapped Cells of Streptococcus salivarius subsp. thermophilus, Lactobacillus delbrueckii subsp. bulgaricus, and Lactococcus lactis subsp. lactis in a Whey Permeate Medium

Author:

Audet Pascal1,Paquin Celine1,Lacroix Christophe1

Affiliation:

1. Groupe de Recherche Stela, Département de Sciences et Technologie des Aliments, Université Laval, Sainte-Foy, Québec, Canada G1K 7P4

Abstract

Cells of Streptococcus salivarius subsp. thermophilus and Lactococcus lactis subsp. lactis entrapped in k -carrageenan-locust bean gum gel performed similarly to free cells in the conversion of lactose to lactic acid. Bead diameter influenced the fermentation rate. Cells entrapped in smaller beads (0.5 to 1.0 mm) showed higher release rates, higher lactose, glucose, and formic acid utilization, higher galactose accumulation, and higher lactic acid production than did cells entrapped in larger beads (1.0 to 2.0 mm). Values for smaller beads were comparable with those for free cells. Immobilization affected the fermentation rate of lactic acid bacteria, especially Lactobacillus delbrueckii subsp. bulgaricus. Entrapped cells of L. delbrueckii subsp. bulgaricus demonstrated a lower lactic acid production than did free cells in batch fermentation. The kinetics of the production of formic and pyruvic acids by L. lactis subsp. lactis and S. salivarius subsp. thermophilus are presented.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference23 articles.

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2. Stabilization of K-carrageenan gel with polymeric amines: use of immobilized cells as biocatalysts at elevated temperature;Chao K. C.;Biotechnol. Bioeng.,1986

3. Choisy C. M. Desmazeaud J. C. Gripon G. Lambert J. Lenoir and C. Tourneur. 1984. Les phenomenes microbiologiques et enzymatiques et la biochimie de l'affinage p. 62-89. In A. Eck (ed.) Le fromage. CNIEL Paris.

4. Intraparticle diffusional effects in immobilized particles;Dalili M.;Appl. Microbiol. Biotechnol.,1987

5. Stability of calciumalginate during citric acid production of immobilized Aspergillus niger;Eikmeier H.;Appl. Microbiol. Biotechnol.,1987

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