Affiliation:
1. Department of Microbiology, College of Medicine, University of Illinois at the Medical Center, Chicago, Illinois
Abstract
There have been few reports on the preservation of viruses at the temperature of liquid nitrogen (-196 C). In this study, factors affecting the survival of phage were observed. Phage lysates in broth did not lose titer after a storage period of 4 weeks in liquid nitrogen. The rate of freezing was not critical, but freezing in nitrogen vapor was not satisfactory. When the virus was partially purified and suspended in buffered saline solution, it rapidly lost titer. Of seven protective agents recommended in the literature, only gum acacia seemed to demonstrate significant protection of purified virus against loss of viability. The temperature of thawing of phage suspended in broth did not affect its titer after freezing and storing in liquid nitrogen.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference10 articles.
1. ADAMS M. 1959. Bacteriophages p. 450-451. Interscience Publishers New York.
2. Experiments on drying and on freezing bacteriophage;CAMPBELL-RENTON M. L.;J. Pathol. Bacteriol.,1941
3. Attempts to freeze some bacteriophages to ultralow temperatures;CLARK W. A.;Appl. Microbiol.,1962
4. Bovine plasma albumin in buffered saline solution as a diluent for viruses;DICK G. W.;J. Immunol.,1949
5. Inactivation of the influenza virus by low voltage roentgen rays;KROEGER A. V.;J. Bacteriol.,1959
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献