Affiliation:
1. American Type Culture Collection, Washington, D.C.
Abstract
A variety of bacteriophages specific for different hosts, including
Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens, Shigella dysenteriae, Staphylococcus aureus
, and
Vibrio comma
, were frozen at a controlled rate to liquid nitrogen temperatures, and then quick-thawed. Glycerol (10%) was used as a protective additive. Quantitative determinations showed from 10% to virtually 100% recovery in the various cases. Host specificity, plaque morphology, and, in one case, rate of inactivation of phage by homologous antiserum remained unchanged.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference8 articles.
1. Methods of study of bacterial viruses;ADAMS M. H.;Methods in Med. Research,1950
2. Classification of bacterial viruses; characteristics of the T5 species and the T2, C,6 species;ADAMS M. H.;J. Bacteriol.,1952
3. AMERICAN TYPE CULTURE COLLECTION. 1958. Catalog of cultures 6th ed.
4. Comparison of several methods for preserving bacteriophage;CLARK W. A.;Appl. Microbiol.,1962
5. HAUMANN W. B. 1960. Low temperature preservation of biological materials and the significant role of liquid nitrogen in the development of frozen semen for the artificial insemination of cattle. Ann. Rept. Compressed Gas Association.
Cited by
7 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献