Cloning and mapping of the genetic determinants for microcin B17 production and immunity

Author:

San Millan J L,Hernandez-Chico C,Pereda P,Moreno F

Abstract

Plasmid pMccB17 (70 kilobases [kb]) codes for the production of microcin B17, a peptide that inhibits DNA synthesis, and for microcin B17 immunity. A BamHI-EcoRI fragment of 5.1 kb from pMccB17 was cloned into pBR322 in two steps. The resulting plasmid (pMM102) overproduced microcin B17 and expressed immunity against microcin. Mcc- and Mcc- Imm- mutants were isolated on plasmids pMccB17 and pMM102 by deleting various DNA fragments and by inserting different translocatable elements. Physical and phenotypic characterization of these mutants showed that a DNA region of 3.0 to 3.5 kb is required to produce microcin B17, whereas an adjacent region of about 1.0 kb is required to express microcin B17 immunity.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference25 articles.

1. A new family of low molecular weight antibiotics from Enterobacteria;Asensio C.;Biochem. Biophys. Res. Commun.,1976

2. Microcin plasmids: a group of extrachromosomal elements coding for low-molecular-weight antibiotics in Escherichia coli;Baquero F.;J. Bacteriol.,1978

3. The microcins;Baquero F.;FEMS Lett.,1984

4. Baquero F. F. SAnchez V. Rubio and A. Tenorio. 1981. Genetical bases of microcin classification p. 578. In S. B. Levy R. C. Clowes and E. L. Koenig (ed.) Molecular biology pathogenicity and ecology of bacterial plasmids. Plenum Publishing Corp. New York.

5. Berg D. 1977. Insertion and excision of the transposable kanamycin resistance determinant Tn 5 p. 205-212. In A. I. Bukhari J. A. Shapiro and S. L. Adhya (ed.) DNA insertion elements plasmids and episomes. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3