14 C Incorporation into the Fatty Acids and Aliphatic Hydrocarbons of Sarcina lutea

Abstract

An initial investigation into the mechanism of hydrocarbon biosynthesis in Sarcina lutea was performed by measuring the amounts of 14 C incorporated into the hydrocarbons and fatty acids by use of a combination gas chromatograph and high-temperature gas-flow ionization apparatus. Uniformly labeled l -isoleucine- 14 C was predominantly incorporated into the anteiso-branched chains. Palmitate- 16 - 14 C gave evidence that a direct correlation may exist between the nonpolar end of the palmitate and the biosynthesis of hydrocarbons and carotenoids. The label from palmitate- 1 - 14 C was incorporated into the various hydrocarbon groups as a compound, derived from the polar end of the palmitate, consisting of more than two carbon atoms. Palmitate- 16 - 14 C and - 1 - 14 C gave no detectable evidence that transformed products were incorporated into other fatty acids. Sodium acetate- 2 - 14 C and uniformly labeled l -leucine- 14 C gave evidence of a nonspecific incorporation into both the aliphatic hydrocarbons and fatty acids of Sarcina lutea .