Affiliation:
1. Physical Defense Division, U.S. Army Biological Laboratories, Fort Detrick, Frederick, Maryland
Abstract
Huston, Charles
K. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.),
and Phillip W. Albro
. Lipids of
Sarcina lutea
. I. Fatty acid composition of the extractable lipids. J. Bacteriol.
88:
425–432. 1964.—The extractable lipids of
Sarcina lutea
were separated into several fractions by a combination of column and thin-layer chromatography. Qualitative and quantitative characterization of the fatty acid content of these lipid fractions was accomplished by means of gas-liquid chromatography and infrared analyses. Of the total extract, the lipids consisted of 2.1% free fatty acids, 51.0% glycerides, and 22.7% complex lipids; they had a fatty acid content with a complete spectrum of carbon numbers from C
8
to C
22
. The fatty acids included a large component of branched-acids in addition to the normal straight-chain acids. The branched-acids, comprising 40% of the fatty acids analyzed, constituted a homologous series of iso-acids from C
12
to C
19
. Two 18-carbon unsaturates were found
cis
-9-octadecenoate and
cis
-11-octadecenoate. A relatively high percentage (20.5%) of the extractable material from
S. lutea
was found to be hydrocarbon. This material was not further characterized.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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