Characterization of the major citrate synthase of Bacillus subtilis

Author:

Jin S1,Sonenshein A L1

Affiliation:

1. Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.

Abstract

The major citrate synthase of Bacillus subtilis (CS-II) was purified to near homogeneity and shown to correspond to the product of the citZ gene. Accumulation of CS-II during exponential growth and stationary phases paralleled expression of the citZ gene. The physical and kinetic properties of CS-II were similar to those of citrate synthase enzymes from Bacillus megaterium and from eukaryotic cells but differed from those of citrate synthases from many gram-negative bacteria.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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4. Relationship between aconitase gene expression and sporulation in Bacillus subtilis;Dingman D. W.;J. Bacteriol.,1987

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