Pectin Lyase Production by a Penicillium italicum Strain-Reference-Cited by-同舟云学术

Pectin Lyase Production by a Penicillium italicum Strain

Published:1989-06 Issue:6 Volume:55 Page:1612-1616
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Alaña Aitor¹,Gabilondo Ane¹,Hernando Fernando¹,Moragues Maria D.¹,Dominguez Juan B.¹,Llama Maria J.¹,Serra Juan L.¹

Affiliation:

1. Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad del País Vasco/Euskal Herriko Unibertsitatea, Apartado 644, E-48080 Bilbao, Spain

Abstract

Growth and concomitant production of an extracellular pectin lyase (PL) [poly(methoxylgalactosiduronate) endolyase; EC 4.2.2.10] were investigated in a group of 16 fungi grown in liquid medium containing pectin as a supplementary carbon source. Culture filtrates of both Penicillium italicum (CECT 2294) and P. expansum (CECT 2275) showed the highest PL activity and contained polygalacturonase but not pectinesterase activity. The effect of the inoculum size, the carbon source (sucrose and glucose syrup), and the presence of pectin on the production of PL by P. italicum was studied. The presence of 2.6 mM glycerophosphate in the culture medium enhanced the appearance of PL but was not inhibitory for the in vitro activity. However, glycerol inhibited the enzyme nearly 50% at such a concentration.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.55.6.1612-1616.1989

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