Extracellular enzyme synthesis in a sporulation-deficient strain of Bacillus licheniformis

Author:

Fleming A B1,Tangney M1,Jørgensen P L1,Diderichsen B1,Priest F G1

Affiliation:

1. Department of Biological Sciences, Heriot Watt University, Edinburgh, United Kingdom.

Abstract

A deletion of the spoIIAC gene of Bacillus licheniformis was prepared in vitro by using the splicing-by-overlap-extension technique. This gene was introduced into B. licheniformis on a temperature-sensitive plasmid, and following integration and excision from the chromosome, a precisely located deletion on the chromosomal gene was prepared. The mutated bacterium was totally asporogenous and formed abortively disporic cells characterized by asymmetric septa at the poles of the cells. Qualitative plate tests revealed that the bacterium synthesized normal levels of DNase, polygalacturonate lyase, protease, RNase, and xylanase, but the hydrolysis zones due to beta-1,3-glucanase and carboxymethyl cellulase activity were smaller in the mutant than in the parent strain. The synthesis of alkaline protease was the same in batch cultures of the mutant and the parent during prolonged incubation for 72 h, but the alpha-amylase yields were reduced by about 30% by the mutation.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference34 articles.

1. Differentiation of mosquito-pathogenic strains of Bacillus sphaericus from non-toxic varieties by ribosomal RNA gene restriction patterns;Aquino de Muro M.;J. Gen. Microbiol.,1992

2. Arbidge M. V. B. A. Balthus J. Schultz and D. Crabb. 1993. Fermentation of Bacillus p. 871-895. In A. L. Sonenshein J. A. Hoch and R. Losick (ed.) Bacillus subtilis and other gram-positive bacteria: biochemistry physiology and molecular genetics. American Society for Microbiology Washington D.C.

3. High efficiency gene inactivation and replacement system for gram-positive bacteria;Biswas I.;J. Bacteriol.,1993

4. Sporulation in Bacillus subtilis. Characterization of oligosporogenous mutants and comparison of their phenotypes with those of asporogenous mutants;Coote J. G.;J. Gen. Microbiol.,1992

5. Deletion of spoIIAB blocks endospore formation in Bacillus subtilis at an early stage;Coppelecchia R.;J. Bacteriol.,1991

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3