Inhibition of Chemoautotrophic Nitrification by Sodium Chlorate and Sodium Chlorite: a Reexamination

Author:

Hynes Russell K.1,Knowles Roger1

Affiliation:

1. Department of Microbiology, Macdonald Campus, McGill University, Ste. Anne de Bellevue, Quebec, Canada H9X IC0

Abstract

The oxidation of NH 4 + by Nitrosomonas europaea was insensitive to 10 mM NaClO 3 (sodium chlorate) but was strongly inhibited by NaClO 2 (sodium chlorite; K i , 2 μM). The oxidation of NO 2 by Nitrobacter winogradskyi was inhibited by both ClO 3 and ClO 2 ( K i for ClO 2 , 100 μM). N. winogradskyi reduced ClO 3 to ClO 2 under both aerobic and anaerobic conditions, and as much as 0.25 mM ClO 2 was detected in the culture filtrate. In mixed N. europaea-N. winogradskyi cell suspensions, the oxidation of both NH 4 + and NO 2 was inhibited in the presence of 10 mM ClO 3 after a 2-h lag period, despite the fact that, under these conditions, ClO 2 was not detected in the filtrate. The data are consistent with the hypothesis that, in mixed culture, NH 4 + oxidation is inhibited by ClO 2 produced by reduction of ClO 3 by the NO 2 oxidizer. The use of ClO 3 inhibition of NO 2 oxidation in assays of nitrification by mixed populations necessitates cautious interpretation unless it can be shown that the oxidation of NH 4 + is not affected.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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