A CRISPR Interference Screen of Essential Genes Reveals that Proteasome Regulation Dictates Acetic Acid Tolerance in Saccharomyces cerevisiae-Reference-Cited by-同舟云学术

A CRISPR Interference Screen of Essential Genes Reveals that Proteasome Regulation Dictates Acetic Acid Tolerance in Saccharomyces cerevisiae

Published:2021-08-31 Issue:4 Volume:6 Page:
ISSN:2379-5077
Container-title:mSystems
language:en
Short-container-title:mSystems

Author:

Mukherjee Vaskar¹^ORCID,Lind Ulrika²^ORCID,St. Onge Robert P.³⁴^ORCID,Blomberg Anders²^ORCID,Nygård Yvonne¹^ORCID

Affiliation:

1. Department of Biology and Biological Engineering, Division of Industrial Biotechnology, Chalmers University of Technology, Gothenburg, Sweden

2. Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden

3. Stanford Genome Technology Center, Stanford University, Palo Alto, California, USA

4. Department of Biochemistry, Stanford University, Palo Alto, California, USA

Abstract

Acetic acid is inhibitory to the growth of the yeast Saccharomyces cerevisiae , causing ATP starvation and oxidative stress, which leads to the suboptimal production of fuels and chemicals from lignocellulosic biomass. In this study, where each strain of a CRISPRi library was characterized individually, many essential and respiratory growth-essential genes that regulate tolerance to acetic acid were identified, providing a new understanding of the stress response of yeast and new targets for the bioengineering of industrial yeast.

Funder

Chalmers Area of Advance Energy

Novo Nordisk Fonden

Royal Swedish Academy of Sciences

Publisher

American Society for Microbiology

Subject

Computer Science Applications,Genetics,Molecular Biology,Modelling and Simulation,Ecology, Evolution, Behavior and Systematics,Biochemistry,Physiology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mSystems.00418-21

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