Affiliation:
1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China
Abstract
ABSTRACT
Clustered regularly interspaced palindromic repeats (CRISPR) and their associated
cas
genes have been demonstrated to regulate self-genes and virulence in many pathogens. In this study, we found that inactivation of
cas9
caused reduced adhesion and intracellular survival of the piscine
Streptococcus agalactiae
strain GD201008-001 and significantly decreased the virulence of this strain in zebrafish and mice. Further investigation indicated that the
regR
transcriptional regulator was upregulated in the Δ
cas9
mutant. As
regR
mediates the repression of hyaluronidase, a critical factor involved in opening the blood-brain barrier (BBB) in mice,
cas9
-mediated repression of
regR
transcription is important for
S. agalactiae
to open the BBB and thereby cause meningitis in animals. This study expands our understanding of endogenous gene regulation mediated by CRISPR-Cas systems in bacteria.
Funder
Natural Science Foundation of Jiangsu Province
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
25 articles.
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