Affiliation:
1. Department of Molecular Microbiology and Immunology L-220, Oregon Health and Science University, Portland, Oregon 97201-3098
Abstract
ABSTRACT
The iron transport-biosynthesis (ITB) operon in
Vibrio anguillarum
includes four genes for ferric siderophore transport,
fatD
, -
C
, -
B
, and -
A
, and two genes for siderophore biosynthesis,
angR
and
angT
. This cluster plays an important role in the virulence mechanisms of this bacterium. Despite being part of the same polycistronic mRNA, the relative levels of transcription for the
fat
portion and for the whole ITB message differ profoundly, the levels of the
fat
transcript being about 17-fold higher. Using S1 nuclease mapping,
lacZ
transcriptional fusions, and in vitro studies, we were able to show that the differential gene expression within the ITB operon is due to termination of transcription between the
fatA
and
angR
genes, although a few transcripts proceeded beyond the termination site to the end of this operon. This termination process requires a 427-nucleotide antisense RNA that spans the intergenic region and acts as a novel transcriptional terminator.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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