Nitrogen Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing

Author:

Schmidt Matthias123,Pearson Allison N.124,Incha Matthew R.124,Thompson Mitchell G.15,Baidoo Edward E. K.12,Kakumanu Ramu12,Mukhopadhyay Aindrila12ORCID,Shih Patrick M.1245,Deutschbauer Adam M.45,Blank Lars M.3,Keasling Jay D.12678ORCID

Affiliation:

1. Joint BioEnergy Institute, Emeryville, California, USA

2. Biological Systems & Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA

3. Institute of Applied Microbiology (iAMB), Aachen Biology and Biotechnology (ABBt), RWTH Aachen University, Aachen, Germany

4. Department of Plant and Microbial Biology, University of California, Berkeley, California, USA

5. Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California, USA

6. Joint Program in Bioengineering, University of California, Berkeley, California, USA

7. Institute for Quantitative Biosciences, University of California, Berkeley, California, USA

8. Department of Chemical and Biomolecular Engineering, University of California, Berkeley, California, USA

Abstract

Understanding the genetic basis of P. putida ’s diverse metabolism is imperative for us to reach its full potential as a host for metabolic engineering. Many target molecules of the bioeconomy and their precursors contain nitrogen.

Funder

Deutsche Forschungsgemeinschaft

U.S. Department of Energy

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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