Affiliation:
1. Microbiology Section, The University of Connecticut, Storrs, Connecticut 06268
Abstract
A simple procedure, based on the rapid filtration and washing of
Frankia
vesicle clusters, was devised for the isolation of
Frankia
strains from alder actinorhizal root nodules. Of 46
Alnus incana
subsp.
rugosa
nodules prepared, 42 yielded isolates. A simple medium containing mineral salts, Casamino Acids, and sodium pyruvate proved to be the most effective for isolation. In general, colonies appeared 6 to 20 days after inoculation. On the basis of hyphal morphology, two distinct types of
Frankia
strains were characterized. Randomly selected isolates were tested for infectivity, and all formed root nodules on
A. glutinosa.
Because of its simplicity and efficiency, the procedure is an improved method for the study of
Frankia
diversity in alder root nodules.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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3. Berry A. and J. G. Torrey. 1979. Isolation and characterization in vivo and in vitro of an actinomycetous endophyte from Alnus rubra Bong. p. 69-83. In J. C. Gordon C. T. Wheeler and D. A. Perry (ed.) Symbiotic nitrogen fixation in the management of temperate forests. Oregon State University Press Corvallis Oregon.
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