Abstract
Xanthomonas campestris is not able to grow in lactose media. The lactose operon from Escherichia coli as part of a mini-Mu phage was integrated at random sites in the chromosome of this bacterium. Clones expressing (beta)-galactosidase were selected. The resulting strain X. campestris 204, is suitable for production of xanthan gum directly from lactose.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference10 articles.
1. ~-Galactosidase gene fusions for analyzing gene expression in E. coli and yeast;Casadaban M. J.;Methods Enzymol.,1983
2. Construction of stable lactoseutilizing X. campestris by chromosomal integration of cloned lac genes using filamentous phage Phi-LF DNA;Fu J.;Appl. Microbiol. Biotechnol.,1992
3. Construction of lactose-utilizing Xanthomonas campestris and production of xanthan gum from whey;Fu J.;Appl. Environ. Microbiol.,1990
4. In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon;Groisman E. A.;Proc. Natl. Acad. Sci. USA,1984
5. Kennedy J. F. and I. J. Bradshaw. 1984. Production properties and application of xanthan p. 319-371. In M. E. Bushell (ed.) Progress in industrial microbiology vol. 19. Elsevier Amsterdam.
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献