Abstract
A simiplified version of bacteriophage DF2 transduction, using the thermosensitive mutant DF2tsC, is described. The transduction frequencies of Rhizobium meliloti markers by DF1tsC are of about 10(-6) or higher. The development of transductants at 34 degrees C does not require the addition of antiserum for phage neutralization.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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