Affiliation:
1. Departments of Genetics and Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA
Abstract
ABSTRACT
The detection of noncoding transcription at multiple enhancers within the mammalian genome raises critical questions regarding whether and how this activity contributes to enhancer function. Here, using
in vivo
analysis of a human growth hormone (
hGH
) transgene locus, we report that activation of a domain of noncoding transcription adjacent to the long-range
hGH-N
enhancer, HSI, is established by the enhancer independent of any interactions with its target promoter. We further demonstrate that the appearance of this enhancer-linked noncoding transcription is temporally and spatially concordant with induction of
hGH-N
in the embryonic pituitary. Finally, we show that the level of transcriptional enhancement of
hGH-N
by HSI is directly related to the intensity of HSI-dependent noncoding transcription and is fully independent of the structure of the locally transcribed RNA. These data extend our understanding of the relationship of long-range enhancer activity to enhancer-dependent noncoding transcription and establish a model that may be of general relevance to additional mammalian loci.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Cited by
32 articles.
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