Affiliation:
1. Unité Biologie Moléculaire du Gène chez les Extrêmophiles, Department of Microbiology, Institut Pasteur, 75015 Paris, France
2. Laboratory of Marine Microbiology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan
Abstract
ABSTRACT
By
in silico
analysis, we have identified two putative proviruses in the genome of the hyperthermophilic archaeon
Aeropyrum pernix
, and under special conditions of
A. pernix
growth, we were able to induce their replication. Both viruses were isolated and characterized. Negatively stained virions of one virus appeared as pleomorphic spindle-shaped particles, 180 to 210 nm by 40 to 55 nm, with tails of heterogeneous lengths in the range of 0 to 300 nm. This virus was named
Aeropyrum pernix
spindle-shaped virus 1 (APSV1). Negatively stained virions of the other virus appeared as slightly irregular oval particles with one pointed end, while in cryo-electron micrographs, the virions had a regular oval shape and uniform size (70 by 55 nm). The virus was named
Aeropyrum pernix
ovoid virus 1 (APOV1). Both viruses have circular, double-stranded DNA genomes of 38,049 bp for APSV1 and 13,769 bp for APOV1. Similarities to proteins of other archaeal viruses were limited to the integrase and Dna1-like protein. We propose to classify APOV1 into the family
Guttaviridae
.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
56 articles.
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