Evaluation of the Vitek Systems Gram-Positive Identification card for species identification of coagulase-negative staphylococci

Author:

Bannerman T L1,Kleeman K T1,Kloos W E1

Affiliation:

1. Department of Microbiology, North Carolina State University, Raleigh 27695-7614.

Abstract

Vitek Systems' Gram-Positive Identification test (GPI) card was evaluated for the ability to identify 12 coagulase-negative Staphylococcus species and subspecies. The bionumber generated from the GPI card was examined for its potential use in epidemiological studies. Results indicated that the GPI card had a high degree of correlation with the conventional methods of identification. The species identified with the greatest accuracy were Staphylococcus epidermidis (92%), S. haemolyticus (95%), S. capitis subsp. capitis (88%), and S. saprophyticus (100%). S. hominis (63%) was identified with the least accuracy. The bionumber was found to have limited epidemiological value because of the frequent occurrence of a few major bionumbers.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference17 articles.

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2. Evaluation of the AutoMicrobic System gram-positive identification card for species identification of coagulase-negative staphylococci;Almeida R. J.;J. Clin. Microbiol.,1983

3. Clinical comparison of the AutoMicrobic System gram-positive identification card, API Staph-Ident, and conventional methods in the identification of coagulase-negative Staphylococcus spp;Grasmick A. E.;J. Clin. Microbiol.,1983

4. Gregson D. D. Gopaul P. Hostetler H. Bialkowska-Hobrzanska and 0. Hammerberg. 1991. Evaluation of the Automicrobic System's ability to speciate and detect oxacillin-resistance in coagulase-negative staphylococci abstr. C-233 p. 381. Abstr. 91st Gen. Meet. Am. Soc. Microbiol. 1991.

5. Staphylococcus epidermidis: a significant nosocomial pathogen;Hamory B. H.;Am. J. Infect. Control,1987

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