Affiliation:
1. McDonnell Douglas Astronautics Company-East, St. Louis, Missouri 63166
Abstract
An automated, computerized system, the AutoMicrobic System, has been developed for the detection, enumeration, and identification of bacteria and yeasts in clinical specimens. The biological basis for the system resides in lyophilized, highly selective and specific media enclosed in wells of a disposable plastic cuvette; introduction of a suitable specimen rehydrates and inoculates the media in the wells. An automated optical system monitors, and the computer interprets, changes in the media, with enumeration and identification results automatically obtained in 13 h. Sixteen different selective media were developed and tested with a variety of seeded (simulated) and clinical specimens. The AutoMicrobic System has been extensively tested with urine specimens, using a urine test kit (Identi-Pak) that contains selective media for
Escherichia coli, Proteus
species,
Pseudomonas aeruginosa, Klebsiella-Enterobacter
species,
Serratia
species,
Citrobacter freundii
, group D enterococci,
Staphylococcus aureus
, and yeasts (
Candida
species and
Torulopsis glabrata
). The system has been tested with 3,370 seeded urine specimens and 1,486 clinical urines. Agreement with simultaneous conventional (manual) cultures, at levels of 70,000 colony-forming units per ml (or more), was 92% or better for seeded specimens; clinical specimens yielded results of 93% or better for all organisms except
P. aeruginosa
, where agreement was 86%. System expansion in progress includes antibiotic susceptibility testing and compatibility with most types of clinical specimens.
Publisher
American Society for Microbiology
Reference6 articles.
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