Development of a method for direct quantification of cytomegalovirus antigenemia by flow cytometry-Reference-Cited by-同舟云学术

Development of a method for direct quantification of cytomegalovirus antigenemia by flow cytometry

Published:1997-10 Issue:10 Volume:35 Page:2665-2669
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Imbert-Marcille B M¹,Robillard N¹,Poirier A S¹,Coste-Burel M¹,Cantarovich D¹,Milpied N¹,Billaudel S¹

Affiliation:

1. Virology Laboratory, Institute of Biology of Nantes University Hospital, France. bmimbert@sante.univ-nantes.fr

Abstract

Cytomegalovirus (CMV) antigenemia was directly detected in polymorphonuclear leukocytes (PMNLs) from transplant recipients by using flow cytometry (FC). Two fixation and permeabilization methods and seven anti-CMV monoclonal antibodies (MAbs) were evaluated. 1C3, SL20, and NEA-9221 MAbs were more efficacious. The antigenemia detection threshold of FC was 0.05% positive PMNLs, and percentages correlated well with DNA viral load and the appearance of clinical symptoms.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.35.10.2665-2669.1997

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