Author:
Gleaves C A,Smith T F,Shuster E A,Pearson G R
Abstract
A commercially available monoclonal antibody directed against an early nuclear protein of cytomegalovirus was used with low-speed centrifugation for the rapid detection of this virus from urine specimens inoculated onto MRC-5 cells. A total of 19 of 162 (11.7%) urine specimens inoculated were positive by both immunofluorescence and peroxidase-antiperoxidase procedures (sensitivity, 100%), whereas only 18 of the samples produced cytopathic effects in conventional cell culture (specificity, 94.7%). All specimens were positive by immunofluorescence and peroxidase-antiperoxidase procedures at 36 h postinfection, whereas an average of 9 days was required for cytopathic effects to develop in cell cultures.
Publisher
American Society for Microbiology
Reference15 articles.
1. Rapid detection and quantitation of human cytomegalovirus in urine through DNA hybridization;Chou S.;N. EngI. J. Med.,1983
2. Corey L. J. Dragavon and D. Benjamin. 1982. Immunoperoxidase staining in the clinical virology laboratory p. 246-253. In R. C. Tilton (ed) Rapid methods and automation in microbiology. American Society for Microbiology Washington D.C.
3. Monoclonal antibodies to cytomegalovirus: rapid identification of clinical isolates and preliminary use in diagnosis of cytomegalovirus pneumonia;Goldstein L. C.;Infect. Immun.,1982
4. Detection of human cytomegalovirus and analysis of strain variation;Huang E. S.;Yale J. Biol. Med.,1976
5. Cytomegalovirus infectivity: analysis of the phenomenon of centrifugal enhancement of infectivity;Hudson J. B.;Virology,1976
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