In Vitro Study of IS Apl1 -Mediated Mobilization of the Colistin Resistance Gene mcr-1

Author:

Poirel Laurent123,Kieffer Nicolas123,Nordmann Patrice1234

Affiliation:

1. Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Fribourg, Switzerland

2. French INSERM European Unit, University of Fribourg (LEA-IAME), Fribourg, Switzerland

3. Swiss National Reference Center for Emerging Antibiotic Resistance, Fribourg, Switzerland

4. University of Lausanne and University Hospital Center, Lausanne, Switzerland

Abstract

ABSTRACT The plasmid-mediated mcr-1 gene encodes a phosphoethanolamine transferase that confers resistance to polymyxins. The mcr-1 gene is associated with insertion sequence IS Apl1 (IS 30 family). In vitro mobilization assays demonstrated the functionality of the composite transposon structure IS Apl1-mcr-1 -IS Apl1 . Transposition generated a 2-bp duplication and occurred in AT-rich DNA regions. This is the first report demonstrating the mobility of the mcr-1 gene by transposition.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

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