Affiliation:
1. Control Laboratories, The Upjohn Company, Kalamazoo, Michigan
Abstract
A rapid direct turbidimetric determination of pantothenic acid with the use of
Saccharomyces carlsbergensis
was developed. With a 5.5-hr incubation, the bioassay demonstrated good dose responses within a range up to 0.04 μg/ml of calcium pantothenate. The multivitamin product assay values obtained by the rapid assay method compare well with the values obtained by the standard USP method. β-Alanine interference was reduced to an insignificant level by the incorporation of asparagine in the assay medium. The Upjohn complex products which require Mylase-P enzyme digestion treatments were also satisfactorily assayed.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
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