Affiliation:
1. Purdue University, Department of Biological Sciences, 201 S. University St., West Lafayette, Indiana 47907
Abstract
ABSTRACT
We describe a genetic system for producing specific gene knockouts in
Cyanothece
sp. strain PCC 7822 using a single-stranded DNA technique (B. Zorin, P. Hegemann, and I. Sizova, Eukaryot. Cell 4:1264-1272, 2005). The first fully segregated mutant was a Δ
nifK
mutant, and it was unable to grow on medium lacking combined nitrogen and produced virtually no hydrogen.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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