Liquid Nitrogen Preservation of Standard Inoculum: Gas-Phase Storage

Author:

Mcdaniel L. E.1,Bailey E. G.1

Affiliation:

1. Institute of Microbiology, Rutgers, The State University, New Brunswick, New Jersey 08903

Abstract

Liquid nitrogen storage was the most satisfactory of several methods tested for supplying standard Streptomyces viridoflavus inoculum for laboratory and pilot plant experimentation. Shake-flask cultures were subdivided into sterile cotton-plugged ampoules and stored in the gas space of a liquid nitrogen refrigerator. There were no detectable changes in viability or in candidin-producing capacity over a 12-month test period. The procedure also proved satisfactory with all other organisms tested.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference13 articles.

1. Cryobiology as viewed by the microbiologist;Burns M. E.;Cryobiology,1964

2. Clark W. A. 1962. The use of low temperatures in preserving some microorganisms and other biological materials p. 285-298. Proc. Low Temp. Microbiol. Symp. Campbell Soup Co. Camden N.J.

3. Long-term preservation of fungus cultures with liquid nitrogen refrigeration;Hwang S.;Appl. Microbiol.,1966

4. Control of stock culture preservation and inoculum build-up in bacterial fermentation;Lincoln R. E.;J. Biochem. Microbiol. Technol. Eng.,1960

5. McCoy E. F. 1954. Selection and maintenance of cultures p. 479-490. In L. A. Underkofler and R. J. Hickey [ed.] Industrial fermentations vol. 2. Chemical Publishing Co. Inc. New York.

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