Pectate Lyase Genes Abundantly Expressed During the Infection Regulate Morphological Development of <i>Colletotrichum camelliae</i> and <i>CcPEL16</i> Is Required for Full Virulence to Tea Plants-Reference-Cited by-同舟云学术

Pectate Lyase Genes Abundantly Expressed During the Infection Regulate Morphological Development of Colletotrichum camelliae and CcPEL16 Is Required for Full Virulence to Tea Plants

Published:2023-02-21 Issue:1 Volume:8 Page:
ISSN:2379-5042
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language:en
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Author:

Jiang Hong¹,Cao Qinghai¹,Wang Xinchao²,Lv Wuyun¹^ORCID,Wang Yuchun¹²

Affiliation:

1. College of Tea Science and Tea Culture, Zhejiang A&F University, Hangzhou, Zhejiang, China

2. Tea Research Institute, Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Hangzhou, Zhejiang, China

Abstract

In this study, we first established a PEG-mediated protoplast transformation system of C. camelliae and used it to investigate the biological functions of seven pectate lyase genes ( CcPEL s) which were abundantly expressed during infection. The results provided insights into the contributions of pectate lyase to mycelial growth, conidial production, appressorium formation, and the pathogenicity of C. camelliae. We also confirmed the secretory function of CcPEL proteins and their role in suppressing Bax-induced cell death.

Funder

Fundamental Research Funds for the Provincial Universities of Zhejiang

Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding-Tea Plant

Scientific Research and Development Foundation of Zhejiang A and F University

Natural Science Foundation of Zhejiang Province

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/msphere.00677-22