Affiliation:
1. College of Tea Science and Tea Culture Zhejiang A & F University Hangzhou 311300 Zhejiang China
2. Tea Research Institute, Chinese Academy of Agricultural Sciences/National Center for Tea Improvement/Key Laboratory of Biology Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs Hangzhou 310008 Zhejiang China
Abstract
SUMMARYTea plant [Camellia sinensis (L.) O. Kuntze], as one of the most important commercial crops, frequently suffers from anthracnose caused by Colletotrichum camelliae. The plant‐specific tau (U) class of glutathione S‐transferases (GSTU) participates in ROS homeostasis. Here, we identified a plant‐specific GST tau class gene from tea plant, CsGSTU45, which is induced by various stresses, including C. camelliae infection, by analyzing multiple transcriptomes. CsGSTU45 plays a negative role in disease resistance against C. camelliae by accumulating H2O2. JA negatively regulates the resistance of tea plants against C. camelliae, which depends on CsGSTU45. CsMYC2.2, which is the key regulator in the JA signaling pathway, directly binds to and activates the promoter of CsGSTU45. Furthermore, silencing CsMYC2.2 increased disease resistance associated with reduced transcript and protein levels of CsGSTU45, and decreased contents of H2O2. Therefore, CsMYC2.2 suppresses disease resistance against C. camelliae by binding to the promoter of the CsGSTU45 gene and activating CsGSTU45. CsJAZ1 interacts with CsMYC2.2. Silencing CsJAZ1 attenuates disease resistance, upregulates the expression of CsMYC2.2 elevates the level of the CsGSTU45 protein, and promotes the accumulation of H2O2. As a result, CsJAZ1 interacts with CsMYC2.2 and acts as its repressor to suppress the level of CsGSTU45 protein, eventually enhancing disease resistance in tea plants. Taken together, the results show that the JA signaling pathway mediated by CsJAZ1‐CsMYC2.2 modulates tea plant susceptibility to C. camelliae by regulating CsGSTU45 to accumulate H2O2.
Funder
Fundamental Research Funds for the Provincial Universities of Zhejiang
National Natural Science Foundation of China
Natural Science Foundation of Zhejiang Province
Subject
Cell Biology,Plant Science,Genetics
Cited by
2 articles.
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