Detection of Minority Variants and Mixed Infections in Mycobacterium tuberculosis by Direct Whole-Genome Sequencing on Noncultured Specimens Using a Specific-DNA Capture Strategy-Reference-Cited by-同舟云学术

Detection of Minority Variants and Mixed Infections in Mycobacterium tuberculosis by Direct Whole-Genome Sequencing on Noncultured Specimens Using a Specific-DNA Capture Strategy

Published:2021-12-22 Issue:6 Volume:6 Page:
ISSN:2379-5042
Container-title:mSphere
language:en
Short-container-title:mSphere

Author:

Lozano Nuria¹²,Lanza Val F.³⁴,Suárez-González Julia¹⁵,Herranz Marta¹²⁶,Sola-Campoy Pedro J.¹²,Rodríguez-Grande Cristina¹²,Buenestado-Serrano Sergio¹²,Ruiz-Serrano María Jesús¹²,Tudó Griselda⁷,Alcaide Fernando⁸⁹^ORCID,Muñoz Patricia¹²⁶¹⁰^ORCID,García de Viedma Darío¹²⁶^ORCID,Pérez-Lago Laura¹²^ORCID

Affiliation:

1. Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain

2. Servicio de Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain

3. Bioinformatics Unit IRYCIS, University Hospital Ramón y Cajal, Madrid, Spain

4. CIBER Enfermedades Infecciosas, Madrid, Spain

5. Unidad de Genómica, Hospital General Universitario Gregorio Marañón, Madrid, Spain

6. CIBER Enfermedades Respiratorias, CIBERES, Madrid, Spain

7. Servei de Microbiologia, Hospital Clinic-CDB, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, Barcelona, Spain

8. Servicio de Microbiología, Hospital Universitario de Bellvitge-IDIBELL, L’Hospitalet de Llobregat, Barcelona, Spain

9. Department of Pathology and Experimental Therapy, University of Barcelona, L’Hospitalet de Llobregat, Barcelona, Spain

10. Departmento de Medicina, Universidad Complutense de Madrid, Madrid, Spain

Abstract

We present a new strategy to identify mixed infections and minority variants in Mycobacterium tuberculosis by whole-genome sequencing. The objective of the strategy is the direct detection in patient sputum; in this way, minority populations of resistant strains can be identified at the time of diagnosis, facilitating identification of the most appropriate treatment for the patient from the first moment.

Funder

MEC | Instituto de Salud Carlos III

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mSphere.00744-21

Reference32 articles.

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4. Mixed Mycobacterium tuberculosis–Strain Infections Are Associated With Poor Treatment Outcomes Among Patients With Newly Diagnosed Tuberculosis, Independent of Pretreatment Heteroresistance

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