Development and Testing of a Microbiological Assay To Detect Residual Effects of Disinfectant on Hard Surfaces

Author:

Mariscal Alberto1,Carnero-Varo Manuel1,Gómez-Aracena Jorge1,Fernández-Crehuet Joaquín1

Affiliation:

1. Department of Preventive Medicine and Public Health, Facultad de Medicina, Universidad de Málaga, E-29071 Málaga, Spain

Abstract

ABSTRACT We describe a glucuronidase bioassay for detecting residual bactericidal activity from the use of disinfectants on hard surfaces; in this assay we used formaldehyde, ethanol, isopropanol, chlorine, and a commercial preparation containing 2-bromo-2-nitro-1,3-propanediol. Chlorine and the commercial preparation showed bactericidal activity (53.5% and 98.2%, respectively) for a week after disinfection.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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