Affiliation:
1. Institut Jacques Monod, CNRS, Université Paris 6, Université Paris 7, F-75251 Paris Cedex 05, France
Abstract
ABSTRACT
Luria-Bertani broth supports
Escherichia coli
growth to an optical density at 600 nm (OD
600
) of 7. Surprisingly, however, steady-state growth ceases at an OD
600
of 0.3, when the growth rate slows down and cell mass decreases. Growth stops for lack of a utilizable carbon source. The carbon sources for
E. coli
in Luria-Bertani broth are catabolizable amino acids, not sugars.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference13 articles.
1. Bachmann, B. J. 1996. Derivations and genotypes of some mutant derivatives of Escherichia coli K-12, p. 2460-2488. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology. ASM Press, Washington, D.C.
2. The Complete Genome Sequence of
Escherichia coli
K-12
3. Fishov, I., A. Zaritsky, and N. B. Grover. 1995. On microbial states of growth. Mol. Microbiol. 15 : 789-794.
4. Kubitschek, H. E. 1974. Constancy of the ratio of DNA to cell volume in steady-state cultures of Escherichia coli B/r. Biophys. J. 14 : 119-123.
5. Lange, R., and R. Hengge-Aronis. 1994. The cellular concentration of the σS subunit of RNA polymerase in Escherichia coli is controlled at the levels of transcription, translation, and protein stability. Genes Dev. 8 : 1600-1612.
Cited by
897 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献