In Vitro Susceptibility of El Tor and Classical Vibrio cholerae Strains to Trimethoprim and Sulfamethoxazole

Author:

Northrup Robert S.12,Doyle Meade A.12,Feeley John C.12

Affiliation:

1. Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20014

2. Laboratory of Bacterial Products, Division of Biologics Standards, National Institutes of Health, Bethesda, Maryland 20014

Abstract

Trimethoprim and sulfamethoxazole were tested individually and in combination against El Tor and classical biotype strains of Vibrio cholerae . Determinations of the minimal inhibitory concentration in liquid media and by an agardilution method showed that classical strains were uniformly more resistant to sulfamethoxazole than were El Tor strains. In agar-dilution studies, trimethoprim was equally active against both biotypes. Combination of the agents produced a synergistic action against all strains tested. Testing for susceptibility to sulfonamide is suggested as a possible method for differentiating between El Tor and classical biotypes of V. cholerae .

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Reference11 articles.

1. Detertmiiinationi of sulfonamide sensitivity of bacteria;Bauer N. W.;Chemotherapia,1964

2. Brumfitt W. M. C. Faiers R. E. Pursell D. S. Reeves and A. R. Turnbull. 1969. Bacter-iological pharmacological and clinical studies with trimethoprim-suLiphon 0amiiide combinations. Postgrad. Med. J. 45(Suppl. ):56-61.

3. Classification of Vibrio cholerae (Vibrio comma), including El Tor Vibrio, by infrasubspecific characteristics;Feeley J. C.;J. Bacteriol.,1965

4. Comparative trial of tetracycline, chlor-a;mphenicol, and trimethoprim-sulphamethoxazole in eraidicationi of Vibrio cltolercie El Tor;Gharagozloo R. A.;Brit. Med. J.,1970

5. A new miiethod foi the differentiation of Vibrio colninm and Vibrio El Tor;Han G. K.;Amiier. J. Hyg.,1963

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