Detection of a novel fimbrial structure on the surface of Salmonella enteritidis by using a monoclonal antibody

Author:

Thorns C J1,Sojka M G1,Chasey D1

Affiliation:

1. Central Veterinary Laboratory, Ministry of Agriculture, Fisheries and Food, Weybridge, Surrey, United Kingdom.

Abstract

A fimbrialike structure expressed on the surface of Salmonella enteritidis was identified by using a monoclonal antibody (69/25) produced against intact S. enteritidis cells. Fimbriae were less than 5 nm in diameter and carried a protein consisting of subunits with a molecular weight of 14,300. No hemagglutinating activity associated with the fimbriae was detected. An epitope on the fimbrial antigen identified by monoclonal antibody 69/25 was expressed by all 58 S. enteritidis strains, 12 of 36 S. dublin strains, and a single strain of S. moscow examined. None of 169 other isolates tested from 17 salmonella serogroups expressed this epitope.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference16 articles.

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4. Fimbriae and haemagglutinating activity in Salmonella, Klebsiella, Proteus and Chromobacterium;Duguid J. P.;J. Pathol. Bacteriol.,1958

5. Purification and characterization of fimbriae for Salmonella enteritidis;Feutrier J.;J. Bacteriol.,1986

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