FtsI and FtsW Are Localized to the Septum in Escherichia coli-Reference-Cited by-同舟云学术

FtsI and FtsW Are Localized to the Septum in Escherichia coli

Published:1998-06 Issue:11 Volume:180 Page:2810-2816
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Wang Lilin¹,Khattar Medhat K.²,Donachie W. D.²,Lutkenhaus Joe¹

Affiliation:

1. Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, Kansas 66216,1 and

2. Institute of Cell and Molecular Biology, University of Edinburgh, Edinburgh EH9 3JR, Scotland2

Abstract

ABSTRACT The localization of FtsI (PBP3), a penicillin-binding protein specifically required for cell division in Escherichia coli , was investigated by immunofluorescence microscopy and found to localize to the septum. The localization of FtsI was not observed in ftsZ or ftsA mutants, indicating that it was dependent on the prior localization of these proteins. Addition of furazlocillin, a specific inhibitor of FtsI, prevented localization of FtsI even though FtsZ and FtsA localization occurred. Interestingly, the localization of FtsN was also prevented by furazlocillin. FtsZ displayed limited localization in furazlocillin-treated cells, whereas it was efficiently localized in FtsI-depleted cells. FtsW, another essential cell division protein, was also localized to the septum.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.180.11.2810-2816.1998

Reference49 articles.

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