Influence of Disk Preparation on Detection of Metallo-β-Lactamase-Producing Isolates by the Combined Disk Assay

Author:

Andrade Soraya S.1,Picão Renata C.2,Campana Eloiza H.2,Nicoletti Adriana G.2,Pignatari Antônio C. C.1,Gales Ana C.12

Affiliation:

1. Laboratório Especial de Microbiologia Clínica, Universidade Federal de São Paulo, São Paulo, Brazil

2. Laboratório ALERTA, Universidade Federal de São Paulo, São Paulo, Brazil

Abstract

ABSTRACT The combined disk assay has been used for detection of metallo-β-lactamase-producing isolates. We have observed that the size of inhibition zones produced by many β-lactam/metallo-β-lactamase inhibitor (IMBL) combinations may differ depending on the way that the combined disks were prepared. Among the 10 β-lactam/IMBL combinations tested, only the imipenem/EDTA combination produced similar results.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference20 articles.

1. Convenient Test for Screening Metallo-β-Lactamase-Producing Gram-Negative Bacteria by Using Thiol Compounds

2. Genetic analysis of a multidrug-resistant (MDR) Enterobacter cloacae producing IMP-1 metallo-β-lactamase (MBL) 2006

3. Discrepancy in the metallo-beta-lactamase phenothypic tests results associated with diversity in the promoter region of class 1 integrons 2006

4. Molecular Characterization of a β-Lactamase Gene, bla GIM-1 , Encoding a New Subclass of Metallo-β-Lactamase

5. Phenotypic Detection of Carbapenem-Susceptible Metallo-β-Lactamase-Producing Gram-Negative Bacilli in the Clinical Laboratory

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