Phenotypic detection of extended spectrum beta lactamase and metallo beta lactamase producers among multidrug resistant Escherichia coli and Klebsiella spp. in urinary tract infections

Abstract

: The incidence of Urinary tract infection (UTI) concomitantly causing the morbidity and mortality in patients with specific risk factors is highly alarming. () and spp., are the most frequently isolated species and considered as highly significant due to their ability to produce Extended spectrum beta lactamase (ESBL) and Metallo beta lactamase (MBL). UTIs caused by bacteria that produces ESBL and MBL are becoming more common, and the ability of diagnostic microbiology laboratories to promptly screen for and identify these resistant infections is crucial.The main objective of my study is to identify and its susceptibility pattern of ESBL and MBL producing and spp., causing UTI.: A total 0f 200 multi drug resistant (MDR) and spp., were screened for ESBL as well as MBL production by phenotypic methods. : From a total of 350 significant UTI cases, 135 are and 65 are spp., remaining are comprised of other bacteria such as Enterococcus faecalis, Staphylococcus aureus, Pseudomonas aeruginosa, Proteus mirabilis. Among 135 isolates 56 found to be multidrug resistant and 42 were ESBL producers and 9 were MBL producers. Among 65 spp., isolates 23 were multidrug resistant and 22 were ESBL producers and 6 were MBL producers.: This study reveals the prevalence of ESBL and MBL producing multidrug resistant and spp., in urinary tract infections as well as their significant role in treatment failure.