High Concentrations of Manganese in Mueller-Hinton Agar Increase MICs of Tigecycline Determined by Etest

Author:

Fernández-Mazarrasa Carlos1,Mazarrasa Olav2,Calvo Jorge1,del Arco Asunción1,Martínez-Martínez Luis13

Affiliation:

1. Service of Microbiology, University Hospital Marqués de Valdecilla

2. University of Cantabria, and Occupational Safety and Health Center, Santander, Spain

3. Department of Molecular Biology, School of Medicine

Abstract

MICs of tigecycline determined by Etest were 4 to 12 times (three ATCC strains) and 2 to 8 times (50 clinical isolates) higher in Mueller-Hinton agar from Merck than in Mueller-Hinton agar from either Oxoid or Difco. This was related to a much higher concentration of manganese in the medium from Merck.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference18 articles.

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2. Tigecycline MIC Testing by Broth Dilution Requires Use of Fresh Medium or Addition of the Biocatalytic Oxygen-Reducing Reagent Oxyrase To Standardize the Test Method

3. Camarena, J. J., R. González, R. Zaragoza, J. C. Navarro, S. Sancho, and J. M. Nogueira. 2007. Variaciones de CMIs por E-test dependientes del tipo de medio Mueller-Hinton II utilizado en rutina frente Acinetobacter baumannii. Abstr. 025, p. 58. Abstr. XII Reunión Sociedad Española Enfermedades Infecc. Microbiol. Clín. (SEIMC). La Coruña, Spain.

4. Performance standards for antimicrobial disk susceptibility. 2006

5. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 2006

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